Biosafety Guide

Biosafety Guide

May 21, 2014

This is a compilation of articles constituting the Biosafety Guide.

Roles and Responsibilities

EH&S Biological Safety (Biosafety) Program
Institutional Biosafety Committee (IBC)
Principal Investigators (PI)
Laboratory Personnel


EH&S directed training
PI/Supervisor directed training

Biological Use Authorizations

Research Requiring IBC Approval
Institutional Biosafety Committee (IBC)
EH&S Biosafety Pre-Review

Regulations and Standards

Medical Waste Management Program
Select Agent Program
Bloodborne Pathogens Program

Risk Assessment

Risk Groups

Risk Mitigation

Engineering controls
Administrative controls
Risk mitigation of Field work

Incident, Exposure and Spill Response

Serious Injuries
Biological and rDNA exposure incidents
Types of Exposures
Composition of a Basic Biohazard Spill Kit
Spills outside the Laboratory Area
Biosafety Level 1 (BSL1) Spills
Biosafety Level 2 (BSL2) and BBP Spills
Biosafety Level 3 (BSL3) Spills
Blood Spills
Spill in a Biological Safety Cabinet (BSC)
Spills in a Centrifuge
Spill of a Biohazardous, Radioactive Material
Reporting and Investigation Requirements

Decontamination of Biologicals

Autoclave Use and Maintenance
Chemical Decontaminants
Physical Methods

Medical Waste Management

What is "Medical Waste"?
Training Requirements
Medical Waste Segregation, Containment and Transport
Liquid Biohazardous Waste
Containment of Medical Wastes at Points of Generation
Location and Access to the UCLA Campus Medical Waste Accumulation Sites

Occupational Health and Medical Surveillance

Hazard Identification and Risk Assessment
Immunization or Prophylaxis
Medical Surveillance
Post-Exposure Prophylaxis and Medical Monitoring

Transport and Shipping